PROJECT 3

Temporal coordination and heart development

The existence of two modes of gene activation, synchronous or stochastic, has so far only been documented in the early blastoderm stage of Drosophila embryo2. The importance of polymerase pausing and synchronous activation has so far only been tested for a fast morphogenetic process, gastrulation4.

This raises the question of whether the importance of transcriptional coordination is unique to fast processes? To answer this, we want to test whether temporal coordination in gene regulation is required for precision in the specification of a slow and late-developing tissue, such as the Drosophila heart.

Our objective is to monitor and alter the transcriptional dynamics of key cardiogenic factors and examine the phenotypic consequences in terms of cell fate decisions.