PROJECT 1

Uncover the mechanisms responsible for transcriptional coordination in the early Drosophila embryo

Our recent promoter swapping experiments followed by Pol II-ChIP or permanganate footprinting assay suggest that minimal promoter sequences (-50, +50bp from the TSS) are sufficient to create de novo pausing in vivo (Lagha et al., 2013).

However, within this 100bp of DNA sequence, the relative importance of their constituting elements is poorly understood. We are therefore investigating the role of various core promoter elements (e.g., TATA and GAGA) in transcriptional dynamics. Live imaging and quantitative analysis on reporter transgenes will be used.